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转基因动物中心简介
    转基因动物中心以亚博足彩转化医学研究院为依托,成立于2011年,拥有近2000平方米的实验空间,其中用于生产和饲养转基因小鼠的百万级洁净鼠房(SPF级动物房)约1000余平方米。
      SPF动物房作为本中心的建设重点,建有万级屏障系统,五个饲养间(包括大小鼠实验室)、胚胎冻存室、显微注射室、动物尸体冻存室、检疫隔离室、洗消间、饲料间、垫料间、配电间等。其基本硬件设施配备完善,目前已经正式进入运行阶段。本中心将致力发展成为江西省首家AAALAC认证的动物中心。
      平台配备两套微注射工作台及其辅助设施、胚胎干细胞培养室、外科手术室、动物饲养设备等。主要开展各种小鼠转基因关键技术的研究和转基因小鼠的生产,包括目的基因过表达、基因敲除、条件性基因敲除小鼠等,为相关疾病研究提供动物模型和新药筛选模型,同时为我省高校及各类科研机构的高水平研究项目提供支撑。
      本中心还拥有大型实验室、细胞房各一间,配备有荧光倒置显微镜、全波长酶标仪、荧光显微镜、荧光体式镜、流式细胞仪、低温高速离心机、血细胞分析仪等实验仪器,以满足科研人员动物实验的需要。
    中心主任邓柯玉教授,曾任美国康奈尔大学动物中心主任,于2009年回国,主要从事大鼠和小鼠转基因动物模型的制备、心血管发育生物学、代谢性疾病研究。目前邓柯玉教授带领的科研团队共有在职博士1人,博士生3人,硕士生7人,实验员2人。
    邓柯玉教授发表的文章:
    [1] Wang, L. F., Wang, X. N., Huang, C. C., Hu, L., Xiao, Y. F., Guan, X. H., Qian, Y. S., Deng, K. Y., and Xin, H. B. (2017) Inhibition of NAMPT aggravates high fat diet-induced hepatic steatosis in mice through regulating Sirt1/AMPKalpha/SREBP1 signaling pathway, Lipids in health and disease 16, 82.
    [2] Feng, J., Huang, P., Shi, S., Deng, K. Y., and Wu, F. Y. (2017) Colorimetric detection of glutathione in cells based on peroxidase-like activity of gold nanoclusters: A promising powerful tool for identifying cancer cells, Analytica chimica acta 967, 64-69.
    [3] Jiang, M. X., Hong, X., Liao, B. B., Shi, S. Z., Lai, X. F., Zheng, H. Y., Xie, L., Wang, Y., Wang, X. L., Xin, H. B., Fu, M., and Deng, K. Y. (2017) Expression profiling of TRIM protein family in THP1-derived macrophages following TLR stimulation, Scientific reports 7, 42781.
    [4] Jiang, M., Deng, K., Jiang, C., Fu, M., Guo, C., Wang, X., Wang, X., Meng, F., Yang, S., Deng, K., Chen, T., and Xin, H. (2016) Evaluation of the Antioxidative, Antibacterial, and Anti-Inflammatory Effects of the Aloe Fermentation Supernatant Containing Lactobacillus plantarum HM218749.1, Mediators of inflammation.
    [5] Yuan, Q., Deng, K. Y., Sun, L., Chi, S., Yang, Z., Wang, J., Xin, H. B., Wang, X., and Ji, G. (2016) Calstabin 2: An important regulator for learning and memory in mice, Scientific reports 6, 21087.
    [6] Guan, X. H., Liu, X. H., Hong, X., Zhao, N., Xiao, Y. F., Wang, L. F., Tang, L., Jiang, K., Qian, Y. S., Deng, K. Y., Ji, G., Fu, M., and Xin, H. B. (2016) CD38 Deficiency Protects the Heart from Ischemia/Reperfusion Injury through Activating SIRT1/FOXOs-Mediated Antioxidative Stress Pathway, Oxidative medicine and cellular longevity 2016, 7410257.
    [7] Wu, L., Luo, M., Deng, R., Liu, T., Deng, K., and Lin, S. (2016) Synthesis and antitumour activity of diorganotin biscarboxylates containing Silicon, Chemical Research and Application 28, 716-719.
    [8] Xue, C., Shi, X., Fang, X., Tao, H., Zhu, H., Yu, F., Ding, X., Liu, M., Fang, F., Yang, F., Wei, Z., Chen, T., Wang, Z., Wang, G., Cheng, X., Wei, J., Lin, Y., Deng, K., Wang, X., and Xin, H. (2016) The "Pure Marriage" between 3D Printing and Well-Ordered Nanoarrays by Using PEALD Assisted Hydrothermal Surface Engineering, ACS applied materials & interfaces 8, 8393-8400.
    [9] Zhan, W., Han, T., Zhang, C., Xie, C., Gan, M., Deng, K., Fu, M., and Wang, J. B. (2015) TRIM59 Promotes the Proliferation and Migration of Non-Small Cell Lung Cancer Cells by Upregulating Cell Cycle Related Proteins, PloS one 10, e0142596.
    [10] Zeng, Y. F., Xiao, Y. S., Lu, M. Z., Luo, X. J., Hu, G. Z., Deng, K. Y., Wu, X. M., and Xin, H. B. (2015) Increased expression of formin-like 3 contributes to metastasis and poor prognosis in colorectal carcinoma, Experimental and molecular pathology 98, 260-267.
    [11] Yuan, Q., Chen, Z., Santulli, G., Gu, L., Yang, Z. G., Yuan, Z. Q., Zhao, Y. T., Xin, H. B., Deng, K. Y., Wang, S. Q., and Ji, G. (2014) Functional role of Calstabin2 in age-related cardiac alterations, Scientific reports 4, 7425.
    [12] 廖启华, 林森, 邓瑞红, 黄志强, 邓柯玉, and 严兆华. (2015) 含硅二烃基锡(Ⅳ)的2-巯基苯并噻唑配合物的合成、结构及抗癌活性, 有机化学 35, 1923-1928.
    [13] Han, R. W., Xu, H. J., Zhang, R. S., Wang, P., Chang, M., Peng, Y. L., Deng, K. Y., and Wang, R. (2014) Neuropeptide S interacts with the basolateral amygdala noradrenergic system in facilitating object recognition memory consolidation, Neurobiology of learning and memory 107, 32-36.
    [14] Xin, H., Deng, K., and Fu, M. (2014) Post-transcriptional gene regulation by RNA-binding proteins in vascular endothelial dysfunction, Science China-Life Sciences 57, 836-844.
    [15] 王丹, 郑怀宇, 时水珍, 贺香勤, 邓柯玉. (2014) 分离纯化小鼠胰岛的方法学研究, 亚博足彩学报(医学版), 5-8,107.
    [16] J. VanEe, P. S., W. Wang, Y. Li, S. Zhang, R. Williams, K.Y. Deng, J. Pillardy, Q. Sun, T. Stelick, J. Spisak, E. Paronett, L. Cote, R. Cameron, J. Zhao, B. Hover, J. Kresovich, Y. Xin, M. Figueroa, R. Shaknovich, S. Monni, U. Unnsteinsdottir, R. Sherwood, C. Ptak, H. Yan, C. Bayles, G. Xia, R. Doran, R. Bukowski, C. Myers, L. Ponnala, S. Stefanov, M. Howard, J. Flaherty, A. Manocchia, E. Dodge, K. Smith, C. Aquadro, D. Lin, A. Melnick, W. Zipfel, P. Soloway, M. Jin, A. Clark, A. Siepel, J.K.C. Rose, G.S. Grills. (2010) Cornell University Life Sciences Core Laboratories Center, J Biomol Tech 21(3 Suppl), S71.
    [17] Tallini, Y. N., Shui, B., Greene, K. S., Deng, K. Y., Doran, R., Fisher, P. J., Zipfel, W., and Kotlikoff, M. I. (2006) BAC transgenic mice express enhanced green fluorescent protein in central and peripheral cholinergic neurons, Physiological genomics 27, 391-397.
    [18] Xin, H. B., Deng, K. Y., Shui, B., Qu, S., Sun, Q., Lee, J., Greene, K. S., Wilson, J., Yu, Y., Feldman, M., and Kotlikoff, M. I. (2005) Gene trap and gene inversion methods for conditional gene inactivation in the mouse, Nucleic acids research 33, e14.
    [19] Ji, G., Feldman, M. E., Deng, K. Y., Greene, K. S., Wilson, J., Lee, J. C., Johnston, R. C., Rishniw, M., Tallini, Y., Zhang, J., Wier, W. G., Blaustein, M. P., Xin, H. B., Nakai, J., and Kotlikoff, M. I. (2004) Ca2+-sensing transgenic mice: postsynaptic signaling in smooth muscle, The Journal of biological chemistry 279, 21461-21468.
    [20] Kotlikoff, M. I., Kannan, M. S., Solway, J., Deng, K. Y., Deshpande, D. A., Dowell, M., Feldman, M., Green, K. S., Ji, G., Johnston, R., Lakser, O., Lee, J., Lund, F. E., Milla, C., Mitchell, R. W., Nakai, J., Rishniw, M., Walseth, T. F., White, T. A., Wilson, J., Xin, H. B., and Woodruff, P. G. (2004) Methodologic advancements in the study of airway smooth muscle, The Journal of allergy and clinical immunology 114, S18-31.
    [21] Rishniw, M., Xin, H. B., Deng, K. Y., and Kotlikoff, M. I. (2003) Skeletal myogenesis in the mouse esophagus does not occur through transdifferentiation, Genesis (New York, N.Y. : 2000) 36, 81-82.
    [22] Xin, H. B., Deng, K. Y., Rishniw, M., Ji, G., and Kotlikoff, M. I. (2002) Smooth muscle expression of Cre recombinase and eGFP in transgenic mice, Physiological genomics 10, 211-215.
    [23] Xin, H. B., Senbonmatsu, T., Cheng, D. S., Wang, Y. X., Copello, J. A., Ji, G. J., Collier, M. L., Deng, K. Y., Jeyakumar, L. H., Magnuson, M. A., Inagami, T., Kotlikoff, M. I., and Fleischer, S. (2002) Oestrogen protects FKBP12.6 null mice from cardiac hypertrophy, Nature 416, 334-338.
    [24] Kume, T., Deng, K., and Hogan, B. L. (2000) Murine forkhead/winged helix genes Foxc1 (Mf1) and Foxc2 (Mfh1) are required for the early organogenesis of the kidney and urinary tract, Development (Cambridge, England) 127, 1387-1395.
    [25] Kume, T., Deng, K., and Hogan, B. L. (2000) Minimal phenotype of mice homozygous for a null mutation in the forkhead/winged helix gene, Mf2, Mol Cell Biol 20, 1419-1425.
    [26] Winnier, G. E., Kume, T., Deng, K., Rogers, R., Bundy, J., Raines, C., Walter, M. A., Hogan, B. L., and Conway, S. J. (1999) Roles for the winged helix transcription factors MF1 and MFH1 in cardiovascular development revealed by nonallelic noncomplementation of null alleles, Developmental biology 213, 418-431.
    [27] Kidson, S. H., Kume, T., Deng, K., Winfrey, V., and Hogan, B. L. (1999) The forkhead/winged-helix gene, Mf1, is necessary for the normal development of the cornea and formation of the anterior chamber in the mouse eye, Developmental biology 211, 306-322.
    [28] Kume, T., Deng, K. Y., Winfrey, V., Gould, D. B., Walter, M. A., and Hogan, B. L. (1998) The forkhead/winged helix gene Mf1 is disrupted in the pleiotropic mouse mutation congenital hydrocephalus, Cell 93, 985-996.
    [29] Zhao, G. Q., Deng, K., Labosky, P. A., Liaw, L., and Hogan, B. L. (1996) The gene encoding bone morphogenetic protein 8B is required for the initiation and maintenance of spermatogenesis in the mouse, Genes & development 10, 1657-1669.
    [30] Deng, K. Y., Wang, D. Y., and Qiu, P. (1993) [Protective effects of tetrandrine on calcium paradox in isolated rat heart], Yao xue xue bao = Acta pharmaceutica Sinica 28, 886-892.
    [31] Xin, H. B., and Deng, K. Y. (1990) [Classification, distribution and functions of 5-hydroxytryptamine receptors], Sheng li ke xue jin zhan [Progress in physiology] 21, 235-241.
    现中心人员结构如下:
    中心设施及对应管理组成包括: